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# ISQ-column Operation Guide
If you are new to ISQ LC-MS and need access to it, please read the following guide.
There will be a video soon (hopefully). For now, please take the **traininig** and
follow **operation procedure** section of this guide.
* PAY SPECIAL ATTENTION !! DO NOT SET BLANK AS TYPE:BLANK, keep the type as UNKNOWN.
* Sample tray can be moved by hand when not injecting.
* Mass-range is predefined to full operational range
* Stay away of pollutants!!
### How to gain access
* Contact your MS tutor.
* Request MS tutor to write me (Jan) and I will grant you access for the booking system.
### Booking system
[Link to the booking system](https://bookings.science.ru.nl/public/portal/index/domain/3/catid/25)
### Known Pollutants
* polar aprotic solvents (DMF, DMSO, HMPA..)
* buffers (TEAB ..) and concentrated ionic/easily ionizable compounds (TEA, DIPEA..)
* electrolytes (TBAF, TBAPF6, ...)
* molecular sieves, microcrystalline compounds
* low-grade solvents
* concentrated samples
* plastics, plasticizers
* triphenylphosphine-oxide
### Operation procedure
* Check if the solvent is there (> 200ml). If not, refill with pre-mix. If pre-mix is gone, contact me (Jan Zelenka, 03.409).
* Copy the standard sample template to your folder and personalize it.
* Set the sample flow low after finishing your sequence (will be automatic in future)
* Write a note into the logbook (its next to the machine, you will see notes from previous users there)
Machine is not connected to the internet at the moment. It will be conected once it will be at its new location.
### When something goes wrong
Contact your MS tutor! If MS tutor is not around contact some other MS tutor.
### List of MS Tutors
|Name | Group |
| ----------- | ----------- |
Your group not in a list? **Become a MS tutor yourself!** (PhD preferred)
### Questions/Misc
* If something is wrong in this guide feel free to contact me.
### TODO (planned improvements)
* SOP is still missing
* Explicitly show the type blank FAQ issue in the video !!